RESUMO
2D-TLC and RP-HPLC methods were applied to qualitatively determinate free phenolic acids and those liberated by acid and alkaline hydrolysis in the flowers and leaves of G. robusta and G. squarrosa. The presence of eleven phenolic acids, namely: caffeic, chlorogenic, p-coumaric, p-hydroxybenzoic, ferulic, gallic, protocatechuic, vanillic salicylic, p-hydroxyphenylacetic and ellagic acids was determined. Quantitative estimate of phenolic acids, expressed as caffeic acid, has been analyzed by the method described in the Polish Pharmacopoeia VIII. The content of phenolic acids in G. robusta reached 7.33 mg/g and 6.23 mg/g for flowers and leaves, respectively. The flowers and leaves of G. squarrosa were characterized by similar level of phenolic acids, namely 6.81 mg/g and 6.59 mg/g, respectively.
Assuntos
Grindelia/química , Hidroxibenzoatos/análise , Calibragem , Cromatografia Líquida de Alta Pressão/normas , Cromatografia em Camada Fina/normas , Flores , Concentração de Íons de Hidrogênio , Hidrólise , Folhas de Planta , Padrões de ReferênciaRESUMO
The qualitative and quantitative analysis of flavonoids, and the quantitative determination of procyanidins in the flowers of naturally growing Pyrus communis and in the flowers of cultivated varieties were carried out. The flavonoid compounds were investigated by chromatographic methods. The flavonoid samples were found in all the studied plant materials. The content of flavonoids was determined by the Christ-Müller's and HPLC methods after acid hydrolysis. The quantitative determination of procyanidins was carried out by the spectrophotometric method.
Assuntos
Biflavonoides , Flavonoides/química , Proantocianidinas , Pyrus/química , Catequina/química , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Flores/química , Solventes , Espectrofotometria Infravermelho , Espectrofotometria UltravioletaRESUMO
The qualitative analysis and quantitative determination of hydroquinone derivatives (arbutin, pyroside) in the flowers of naturally growing pear tree and of its four cultivated varieties (Pomaranczówka, Lukasówka, Klapsa, Salisbury) were carried out. The comparative qualitative analysis of hydroquinone derivatives was investigated chromatographically (TLC). Arbutin and pyroside were found in all the studied plant materials and free hydroquinone was found in buds of naturally growing pear. The content of arbutin and pyroside was determined by the HPLC method.
Assuntos
Hidroquinonas/química , Pyrus/química , Arbutina/química , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Flores/química , Hidroquinonas/isolamento & purificação , Padrões de Referência , Solventes , Espectrofotometria UltravioletaRESUMO
Five flavonoid glycosides: kaempferol 3-O-beta-D-glucopyranoside (IV), isorhamnetin 3-O-beta-D-glucopyranoside (V), isorhamnetin 3-O-beta-D-(6''-O-alpha-L-rhamnopyranosyl)-glucopyranoside (VIII), 8-methoxykaempferol 3-O-beta-D-(2''-O-alpha-D-glucopyranosyl)-glucopyranoside (VI), 8-methoxykaempferol 3-O-beta-D-(2''-O-alpha-L-rhamnopyranosyl)-glucopyranoside (VII) and chlorogenic acid (IX) were isolated from the flowers naturally growing Pyrus communis L. They were identified by chemical and spectroscopic methods.
